Assisted hatching (AH) is a lab technique that was first suggested in the 1980s. It was developed after fertility experts observed that embryos with a thin zona pellucida (shell) had a higher rate of implantation during in vitro fertilization (IVF).
Assisted hatching is generally performed on the third day of embryo development. The embryologists use a laser to create a very small hole in the zona pellucida. Assisted hatching can also be done on previously frozen and thawed embryos.
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